| John Goodman | Sullivan Truck (voice) |
| Bonnie Hunt | Sally Carrera (voice) |
| Katherine Helmond | Lizzie (voice) |
| Billy Crystal | Mike Car (voice) |
| Edie McClurg | Minny (voice) |
| Jack Angel | Additional Voices (voice) |
| Cheech Marin | Ramone (voice) |
| Tony Shalhoub | Luigi (voice) |
| Owen Wilson | Lightning McQueen (voice) |
| Michael Keaton | Chick Hicks (voice) |
| Jeremy Piven | Harv (voice) |
| Tom Hanks | Woody Car (voice) |
| Paul Dooley | Sarge (voice) |
| Bob Peterson | Additional Voices (voice) |
| Joe Ranft | Red/Peterbilt (voice) |
| Andrew Stanton | Fred (voice) |
| George Carlin | Fillmore (voice) |
| Teddy Newton | Additional Voices (voice) |
| Lou Romano | Snotrod (voice) |
| John Ratzenberger | Mack/Hamm Truck/Abominable Snow Plow/P.T. Flea Car (voice) |
| Jennifer Darling | Additional Voices (voice) |
| Jenifer Lewis | Flo (voice) |
| Richard Kind | Van (voice) |
| Bob Bergen | Additional Voices (voice) |
| Dave Foley | Flik Car (voice) |
| Rodger Bumpass | Additional Voices (voice) |
| Sherry Lynn | Additional Voices (voice) |
| Mickie McGowan | Additional Voices (voice) (as Mickie T. McGowan) |
| Richard Petty | The King (voice) |
| Paul Eiding | Additional Voices (voice) |
| Bill Farmer | Additional Voices (voice) |
| Teresa Ganzel | Additional Voices (voice) |
| Danny Mann | Additional Voices (voice) |
| Laraine Newman | Additional Voices (voice) |
| Guido Quaroni | Guido (voice) |
| Jan Rabson | Additional Voices (voice) |
| Bob Scott | Additional Voices (voice) |
| A.J. Riebli | Additional Voices (voice) (as A.J. Riebli III) |
| Stephen Schaffer | Additional Voices (voice) |
| Jay Leno | Jay Limo (voice) |
| Paul Newman | Doc Hudson (voice) |
| Larry The Cable Guy | Mater (voice) |
| Michael Wallis | Sheriff (voice) |
| Bob Costas | Bob Cutlass (voice) |
| Darrell Waltrip | Darrell Cartrip (voice) |
| H.A. 'Humpy' Wheeler | Tex (voice) (as Humpy Wheeler) |
| Tom Magliozzi | Rusty Rust-eze (voice) |
| Ray Magliozzi | Dusty Rust-eze (voice) |
| Lynda Petty | Mrs. The King (voice) |
| Dale Earnhardt Jr. | Junior (voice) |
| Michael Schumacher | Michael Schumacher Ferrari (voice) |
| Mario Andretti | Mario Andretti (voice) |
| Sarah Clark | Kori Turbowitz (voice) |
| Mike Nelson | Not Chuck (voice) (as Mike 'No Name' Nelson) |
| Jonas Rivera | Boost (voice) |
| Adrian Ochoa | Wingo (voice) |
| E.J. Holowicki | DJ (voice) |
| Elissa Knight | Tia (voice) |
| Lindsey Collins | Mia (voice) |
| Larry Benton | Motorhome Race Fan (voice) |
| Douglas Keever | Motorhome Race Fan (voice) (as Douglas 'Mater' Keener) |
| Tim Allen | Buzz Lightyear Car (voice) |
| Michael Bell | Additional Voices (voice) |
| Susan Blu | Additional Voices (voice) |
| Andrea Boerries | Additional Voices (voice) |
| Marco Boerries | Additional Voices (voice) |
| Torbin Xan Bullock | Additional Voices (voice) |
| Richard Cawood | Additional Voices (voice) |
| Scott Clark | Additional Voices (voice) |
| Kathy Coates | Additional Voices (voice) |
| John Cygan | Additional Voices (voice) |
| Brian Fee | Additional Voices (voice) |
| Craig Good | Additional Voices (voice) |
| Jess Harnell | Additional Voices (voice) |
| Artie Kempner | Additional Voices (voice) |
| Hooman Khalili | Additional Voices (voice) |
| Konishi Sonoko | Additional Voices (voice) (as Sonoko Konishi) |
| Erik Langley | Additional Voices (voice) |
| Colleen O'Shaughnessey | Additional Voices (voice) |
| Steve Purcell | Additional Voices (voice) |
| Dan Scanlon | Additional Voices (voice) |
| Ken Schretzmann | Additional Voices (voice) |
| Matt Staudt | Additional Voices (voice) |
| Jay Ward | Additional Voices (voice) |
| Jim Ward | Additional Voices (voice) |
| Colette Whitaker | Additional Voices (voice) |
